- Development of diagnostic antibodies using the classical hybridoma or the B cell PCR technologies
- Isolation of B cells and cultivation
- Single cell sorting of B cell specific populations
- Screening for target-specific antibodies
- RNA-isolation from B cell lysates and subsequent cDNA synthesis
- RT-PCR of IgG heavy chain and light chain coding sequences and cloning in suitable expression vectors, transformation of E. coli, preparation of constructs
- Process steps with extensive use of automated systems at high throughput
- Work on research projects to support antibody development optimization
- Independent planning of your experiments
- Documentation and presentation of your results
- Master Degree in Biology, Immunology, Biotechnology or a comparable study program
- Knowledge and hand-on experience about basic immunology and cell biology techniques (cell culture, FACS, ELISA)
- Experience in molecular biological methods RT-PCR, cloning) and knowledge in respective analytical methods
- Very interested in laboratory automation
- Curious and open for new ideas
- Not afraid of thinking outside the box
- Excellent English skills, German knowledge is an advantage
- Engagement, strong self-reliance and organizational skills, and very important enjoy working together in a team
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Master Molecular Biology - Penzberg, Deutschland - Roche
Beschreibung
The Position
Your department
Your new position will be located in the 'Antibody Development' subchapter, which belongs to 'Reagent Research & Design' department in Roche Diagnostics.
We aim to improve everybody's health by the design & development of high quality novel reagents and diagnostics solutions. We count on the scientific excellence and innovative solutions of our people and foster a culture of trust, empowerment and cross-functional collaboration.
We aim to develop the best in class antibodies for highly sensitive and specific detection of any biomarker in any test format.
Your Team
We develop antibodies for various platforms of Roche Diagnostics. You will work equally in the groups of 'Antibody Development' and 'Antibody Molecular Biology' that are responsible for the generation of diagnostic antibodies.
After a profound introduction to the techniques and tools for antibody generation, sequencing and recloning, you will work on current projects in the field of personalized healthcare or other exciting projects in the fields of infectious diseases, fertility, oncology or cardiovascular diseases.
Your objectives/tasks
If you have the following experience and knowledge, you have come to the right place:
This position is limited to 2 years.
Application documents
To make the application process as convenient as possible for you please add only your updated CV . References and certificates are not required at this stage.
We look forward to receiving your application
Your