Arqam Mahmood Fazal

It is with great enthusiasm that I present myself as a candidate. I am excited to contribute to your projects. My credentials make me the perfect candidate for this job position. I have done a master degree in Molecular Biology and Evolution from University of Kiel, Germany. My master’s thesis research relates to the extraction and characterization of lipoproteins in microbes using qualitative and quantitative assays, chromatographic and spectroscopic techniques. My research experience involves the implementation of molecular cloning techniques, screening and characterization of biomolecules and doing biochemical assays. I have prior experience in in situ hybridization while working on Fruit fly drosophila melanogaster  as a model for human diseases. I have done an Introductory research module at Institute for Botanical Genetics and Molecular Biology, CAU Kiel under Prof. Frank Kempken. During this research internship, I researched the method to establish CRISPR-Cas9 as a genome-editing tool for Neurospora crassa. I carried out genomic integration of Cas9 gene sequence in the N. crassa genome under the control of a regulatory promoter and creation of DNA expression cassettes in cloning vectors. I achieved the transformation and integration of nit6 promoter in pMM-536 plasmid by restriction endonucleases and DNA ligase. I also did an Advanced research module at Max Planck Institute for Evolutionary Biology, Plön, Germany. There I engaged in quality read mapping and analysis of mouse genome transcriptomes for the mutant gene Peg13 using RNA Seq data under the supervision of Prof. Diethard Tautz.

I have performed PCR, Viral RNA extraction ELISA, Bradford, and immunohistochemistry assays for protein quantification and interactions in Drosophila melanogaster. During my thesis, I extracted and characterized the Biosurfactant molecules. I am familiar with different protein purification methods like, Extraction, Precipitation, Solubilisation, Ultracentrifugation, and chromatographic techniques. I have considerable experience designing plasmid vectors on Clone Manager. I also did statistical and graphical analysis on GraphPad Prism involving data visualizations. I have adequate knowledge of statistical programming languages R and Python.

Since January 2022, I am working at a biotechnology company in Kiel, Germany. I do colony count measurements on Plate count Agar (PCA) and various other culture mediums. I also perform viral DNA and RNA isolation and qPCR for Salmonella, Hepatitis A and Norovirus. I have described my daily tasks in detail in my resume. My earlier experience and my drive to learn new concepts and techniques will make me a valuable member of your team. I think R&D is the fundamental driving force towards scientific advancement and innovation. I am a self-starter, proactive, reliable, and eager to lead. I am fluent in English and have conversational understanding of German. 
 

I am working at a biotechnology company in Kiel, Germany. I do colony count measurements on Plate count Agar (PCA) and various other culture mediums. I also perform viral DNA and RNA isolation and qPCR for Salmonella, Hepatitis A and Norovirus.

I have done an Introductory research module at Institute for Botanical Genetics and Molecular Biology, CAU Kiel under Prof. Frank Kempken. During this research internship, I researched the method to establish CRISPR-Cas9 as a genome-editing tool for Neurospora crassa. I carried out genomic integration of Cas9 gene sequence in the N. crassa genome under the control of a regulatory promoter and creation of DNA expression cassettes in cloning vectors. I achieved the transformation and integration of nit6 promoter in pMM-536 plasmid by restriction endonucleases and DNA ligase. I also did an Advanced research module at Max Planck Institute for Evolutionary Biology, Plön, Germany. There I engaged in quality read mapping and analysis of mouse genome transcriptomes for the mutant gene Peg13 using RNA Seq data under the supervision of Prof. Diethard Tautz.