Angebotene Dienstleistungen €20 / Stunde
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Über Raminder Singh:
I am a highly qualified and professional Senior Scientist and Researcher with extensive qualifications, professional development, and associated experience in laboratory environments. As a result, I have harvested a wide spectrum of scientific and technical skills, including designing assay protocols for primary cell isolation, cell imaging, and biomaterials for 3D tissue printing. Additionally, I have shared my knowledge and expertise through mentorship and supervision of academic students at various levels, including PhD (Biology/Cell Study), MD, MSc, and BSc. I have additionally completed an intensive course called Clinical Research (CRA), where I obtained in-depth learning on: Pharmaceutical management, Medical basics & Clinical research.
With strong scientific and analytical abilities, I have positively led end-to-end projects and tasks, meeting strict deadlines and maintaining regulatory standards and compliance. To date, I have developed a depth of knowledge in cell biology and molecular biology, and I pride myself on my analytical approach that supports process improvements, system implementations and laboratory activities.
An example of my research experience includes cardiovascular diseases, based on the fact they are the leading cause of death worldwide. In patients with contra-indication for stent placement, bypass surgery is the method of choice. Vessels used for surgery can be of autologous or allogenic origin. Autologous vessel procurement reduces the risk of rejection but requires a second surgery site. Additionally, the availability of vascular autografts is limited due to various reasons such as age of patient, status of the vascular tree, pre-surgeries, or size-mismatches. Commercially available synthetic grafts made from polytetrafluoroethylene (PTFE, brand name Teflon) or Darcon are used for large diameter vessel replacement, but their patency is very low due to a limited endothelization leading to thrombosis, hyperplasia, and a lacking integration into the surrounding tissue. This calls for intensifying the research to produce artery-like vessel substitutes. Despite profound progress in the field of artificial blood vessel design, development of sustainable small diameter vessels has not been achieved yet.
I am well versed in building and sustaining relationships with other scientific professionals, both internally and externally. I am a highly motivated person, with exceptional multi-tasking, analytical and organisational skills, and I enjoy working with people on all levels, adopting a critical approach to solving problems and developing scientific processes.
I am looking to improve my networks and remain visible for new work opportunities. I look forward to connecting with you!
- Advanced training course "Gene technology, Biosafety and Biosecurity" for representatives for biological safety according to §15 Abs. 2 Satz 1 Nr.3 GenTSV; Medical School, Hannover (MHH), Germany
- Completed "Basic in laboratory animal science and management perioperative management", (FELASA B) course to responsible for carrying out experimental procedures on animals, MHH, Germany
- Qualified for "National Eligibility Test (NET)" of Agricultural Scientists Recruitment Board (ASRB) with specialization of biotechnology for selection in prestigious Indian research institutes
- Synthesis various Hydrogels using natural material, 2015 to 2020
- Cell Studies with respect to Hydrogels, 2015 to 2020
- Magnetic Loading of the Cells, 2015 to 2020
- Isolation of the smooth muscle & endothelial cells from umbilical cords, 2015 to 2020
- Immunofluorescence Techniques, 2013 to 2020
- Microscopic Study of Cells, 2013 to 2020
- Cell Culture Techniques, 2007 to 2020
|Molecular Biology (15)||Cell (15)||Cell Culture (14)|
|Protein Purification (12)||Western Blotting (11)||Biochemistry (10)|
|Cell Biology (9)||Protein Chemistry (8)||Stem Cells (8)|
|Protein Expression (7)||Molecular Cloning (7)||Cancer (7)|
|Biotechnology (6)||Drug Discovery (5)||PCR (5)|
|ELISA (3)||Enzyme Assays (2)||Polymerase Chain Reaction (PCR) (1)|
|Cell Signalling||Protein Assays||Assay Development|
|Immunoprecipitation||Reverse Transcription Polymerase Chain Reaction (RT-PCR)|
|Tissue Culture||Tissue Engineering||Regenerative Medicine|
|Biomedical Sciences||Biomedical Research|
|Cancer Research||Cancer biology||Cancer stem cells|
|AML||Biomaterials||Tissue Culture: Cell separation from blood (1)|
|Maintenance and Storage of Mammalian Cells (1)||Proliferation and Cell Death Elisa Assay||Transfection, Colony Forming Cell (CFC) Assay (Methylcellulose Assay)|
|Nucleic Acid: Isolation, purification, cloning, colony hybridization||Conjugational Plasmid transfer, Electroporation, SiRNA||ShRNA, Southern Blotting, Primer designing, RT-PCR, PCR|
|Protein: Purification, immune-precipitations, western blotting||Immunohistochemistry: In-situ Hybridization, Immuno-florescence||Bioinformatics: Sequence Analysis and Database Searching – BLAST|
|Leadership||Mentoring||Analysis & Validation|
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