Phd Student - Essen, Deutschland - Deutsches Krebsforschungszentrum

Deutsches Krebsforschungszentrum

Geprüftes Unternehmen

Essen, Deutschland

vor 3 Wochen

Geschrieben von:

Lena Wagner

beBee Recruiter

Beschreibung

Position:
PhD Student - Assay Development for the AHR Signature in Dermatology**
Department: DKTK partner site Essen / Düsseldorf - Klinik für Dermatologie

Code number:

The German Cancer Research Center is the largest biomedical research institution in Germany. With more than 3,000 employees, we operate an extensive scientific program in the field of cancer research.

Together with university partners at seven renowned partner sites, we have established the German Cancer Consortium (DKTK).

At the partner site of DKTK Essen / Düsseldorf the German Cancer Research Center is offering a PhD position for assay development for AHR signature in dermatology.

The PredictAHR project utilizes existing data from the German Cancer Consortium of the tumor entities malignant melanoma, glioma, sarcoma, lung cancer and chronic lymphocytic leukemia and prospectively collects samples that are profiled using high-throughput methods.

Our goal is to identify a reduced set of AHR activity markers that are specific to the different tumor entities and allow the development of simple assays for the clinic.

Translating the transcriptional AHR signature to proteomics data will enable immunohistochemical evaluation by pathology laboratories, while extension to metabolomics data may enable translation to biological fluids such as blood and urine that can be obtained without surgical intervention.

In summary, PredictAHR will investigate the predictive power of the AHR signature in various cancers and develop assays for AHR activity that can be integrated into the clinic.

Job description:

Cell culture: thaw, culture, split, freeze cells; functional in vitro analyses
Molecular biology / protein chemistry: PCR, RT-PCR, qRTPCR, DNA and RNA isolation, protein isolation, Western blots
Primary animal experimental research on tumor metastasis in a translational research project that also includes human experimental issues in patients with malignant melanoma
Immunohistology / immunofluorescence: paraffin / cryoembedding, preparation of slides on microtome, photography of stains on microscope, processing of images on computer
Molecular and flow cytometric study of tumors
Planning and execution of metabolomics and lipidomics measurements, among others, for the characterization of metastases of malignant melanoma
Development of new methods for the characterization of metabolites and lipids using modern multidimensional methods (e.g. LC-LCMS)
Planning and execution of cell culture experiments
Statistical evaluation of results
Documentation and evaluation of research results as well as preparation of publications, lectures, posters
Preparation of the dissertation within the PhD project

Requirements:

University degree (Master) in chemistry, food chemistry or biochemistry of at least 8 semesters
Animal experimental experience (breeding / keeping immunodeficient and immunocompetent mouse lines) and knowledge of basic immunological and molecular biology techniques
Proficiency in complex multicolor flow cytometry experiments and cell sorting
Profound knowledge of analytical chemistry (especially in the fields of LC, GC and MS)
Knowledge of metabolite and lipid analysis as well as bioinformatics is desirable

We offer:

Interesting, versatile workplace
International, attractive working environment
Campus with modern stateoftheart infrastructure
Access to international research networks
Doctoral student payment including social benefits
Flexible working hours
Comprehensive training and mentoring program through the Helmholtz International Graduate School

Important notice:

Earliest Possible Start Date:
as soon as possible

Duration:
The position is limited to 3 years with the possibility of prolongation.

Application Deadline:

Contact:

Sabine Kaul
Phone /

The DKFZ is committed to increase the proportion of women in all areas and positions in which women are underrepresented.

Qualified female applicants are therefore particularly encouraged to apply.