Phd Position - Heidelberg, Deutschland - Universität Heidelberg

Universität Heidelberg

Geprüftes Unternehmen

Heidelberg, Deutschland

vor 2 Wochen

Geschrieben von:

Lena Wagner

beBee Recruiter

Beschreibung

We are seeking a motivated PhD student with a background in biochemistry, cell biology or molecular biology.

The nuclear pore complex (NPC) is a large cylindrical structure with multiple copies of over 30 different proteins named nucleoporins (NUPs).

The NPC is embedded in the nuclear envelope (NE) at fusion sites of the inner and outer nuclear membrane where it facilitates nuclear-cytoplasmic transport of RNA and proteins.

NPCs assemble in the intact nuclear envelope (interphase human cells or yeast cells with a closed mitosis) by an inside-out mechanism.

NUPs become deposited from within the nucleus to the inner nuclear membrane, deform this membrane and eventually the inner and outer nuclear membranes fuse.

We recently have identified with Brr6 and Brl1 two conserved integral membrane proteins that may play a role in nuclear membrane fusion during NPC biogenesis.

In this project we will study NPC biogenesis using budding yeast and human cells as model systems.

The PhD student will use biochemical approaches (Brr6 and Brl1 reconstitution into liposomes), super resolution microscopy (MinFlux), electron microscopy, CRISPR/Cas9 technology and live cell imaging to study NPC assembly.

We offer:

The PhD position is funded in the first instance for 3 years with starting date based on mutual agreement (possible from March/April The remuneration is based on TV-L E13.

Application deadline
:30 April 2024
**Interested?